Disseminated Mycobacterium avium subsp. avium infection in a Captive Richardson's Ground Squirrel (Spermophilus richardsonii)

Abstract 

Although mycobacteria have been isolated from rodents, overt mycobacteriosis is rare in any rodent species. A pet adult male Richardson's ground squirrel (Spermophilus richardsonii) died after a short course of alopecia, anorexia, and weight loss. Necropsy and subsequent histopathological examination of tissue samples revealed disseminated granulomatous inflammation involving the lungs, lymph nodes, liver, intestine, adrenal gland, spleen, pleura, and peritoneum. Ziehl-Neelsen stain revealed high numbers of acid-fast bacilli within the cytoplasm of macrophages and multinucleated giant cells. Polymerase chain reaction performed on paraffin-embedded tissues was positive for Mycobacterium avium subsp. avium and negative for M. bovis-tuberculosis complex. Three females housed with this squirrel remained clinically healthy, and mycobacterial cultures of pooled feces from these animals were negative. To the authors' knowledge, this is the second report of disseminated mycobacteriosis in squirrels involving M. avium subsp. avium, with both cases described recently in the Iberian Peninsula.

Key words: Adrenalitis, mycobacteriosis, Mycobacterium avium subsp. avium, rodent, Spermophilus, squirrel